TITLE:
Recombinant IgM expression in mammalian cells: A target protein challenging biotechnological production
AUTHORS:
Alexander Mader, Veronika Chromikova, Renate Kunert
KEYWORDS:
IgM; Mammalian Expression; Recombinant Antibody Expression; Antibody Purification
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.4A,
April
30,
2013
ABSTRACT: For many years, the
potential of immunoglobulin M (IgM) antibodies was not fully understood because
of characteristics different to the well-known immunoglobulin G type like low
target affinity, cross reactivity and complex protein structure. In the
meanwhile IgMs have been positively evaluated for their use as therapeutic
agent in the mucosal environment but also in serum to eradicate upcoming tumor
cells and invading antigens. Therefore IgM class of antibodies will play a
significant role in clinical applications but also diagnosis in the future. To
evaluate the full potential of this kind of antibody molecules large amounts
of high quality product will be needed. In this review the focus is set on the
biotechnological aspect of producing IgM class antibodies recombinantly in
mammalian cells. Current achievements in expression and purification of this
molecule are highlighted and compared.