TITLE:
Neural differentiation of allogenic mixed-cultured rat bone mesenchymal stem cells
AUTHORS:
Jun Zhou, Huilin Yang, Jiannong Cen
KEYWORDS:
Bone Mesenchymal Stem Cells; Neural Differentiation; Nestin; Neuron Specific Enolase; Glial Fibrillary Acidic Protein
JOURNAL NAME:
Journal of Biomedical Science and Engineering,
Vol.6 No.4,
April
29,
2013
ABSTRACT: Many
studies showed that bone mesenchymal stem cells (BMSCs) can transdifferentiate
to neural cell in vitro. The purpose
of this study was to investigate the mixed-culture of allogenic rat BMSCs in vitro, and to perform neural
differentiation potential characterization. Rat BMSCs were isolated and
cultured by plastic adherence and density gradient centrifugation respectively,
and the 3rd passage cells were harvested and mixed-cultured with same
concentration. The second passage cells of the mixed-cultured cells were
obtained to perform Wright-Gemsa staining for morphological observation, to
identify the surface marker of CD29, CD45, CD90 by flow cytometry and induced
to differentiate into neural cell, then performed immunocytochemistry of
Nestin, NSE and GFAP. Results showed that after Wright-Giemsa staining, the
mixed-cultured cells displayed typical spindle-shape, presented layered and
whirlpool-like growth; and the mixed-cultured
cells were positive for CD29 and CD90, but negative for CD45 by flow
cytometry. After induction, the mixed-cultured cells appeared morphological
changes of neuron and glial cell, and were positive expression of Nestin,
neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP). The
results demonstrated that the mixed-cultured allogenic rat BMSCs can be
induced into neural cell such as neuron and glial cell in vitro.