TITLE:
Determination of 17α-Methyltestosterone in Freshwater Samples of Tilapia Farming by High Performance Liquid Chromatography
AUTHORS:
Isabel R. Barbosa, Sara Lopes, Rhaul Oliveira, Inês Domingues, Amadeu M. V. M. Soares, António J. A. Nogueira
KEYWORDS:
17α-Methyltestosterone; High Performance Liquid Chromatography; Environment; Tilapia Farming
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.4 No.4,
April
29,
2013
ABSTRACT: 17α-methyltestosterone is used to induce the sex reversal of Tilapia sp. to obtain cultures mono-sex to an economically viable. This practice may lead to environmental
contamination and problems in human health. Therefore methods need to be developed to detect residues of 17α-methyltestosterone in aqueous
matrices. A simple high-performance liquid chromatographic method using ultraviolet
detection (245 nm) and testosterone as internal standard has been developed for
the monitoring 17α-methyltestosterone
in freshwater samples of tilapia aquaculture. The method described involves
limited sample preparation as it includes a filtration followed by a single solid-phase extraction step using C18 cartridge.
Validation data indicated that the HPLC-UV method for 17α-methyltestosterone determination in the concentration range of 50 - 2000 μg/L provided good linearity, sensitivity, accuracy and precision.
Method performance was efficiently
applied to monitoring the freshwater samples of fish ponds and the surrounding aquatic channels.