TITLE:
Down Regulation of MyD88 in Macrophages Treated with Liposomes Composed of Phosphatidylserine
AUTHORS:
Yuka Takasugi, Futoshi Kurai, Issei Kazume, Masaki Otsuka, Yoichi Negishi, Rui Tada, Yukihiko Aramaki
KEYWORDS:
Phosphatidylserine; Liposome; MyD88; TGF-β; Macrophage
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.4 No.2,
April
28,
2013
ABSTRACT: We have recently demonstrated that liposomes
composed of phosphatidylserine (PS-liposomes) suppressed nitric oxide and
inflammatory cytokine productions following LPS stimulation in macrophages. In
this study, we examined the effect of PS-liposomes on expressions of TLR-4 and
MyD88, which are essential for the signal transduction in LPS stimulation. Expression
of MyD88 was suppressed when macrophages were treated with PS-liposomes, but
not with liposomes of phosphatidylcholine. No change in TLR-4 expression was
observed. MyD88 suppression was restored to the control levels when cells were
pre-treated with anti-TGF-β antibody, suggesting that TGF-β plays an important role in down-regulation of MyD88
following PS-liposome treatment.