TITLE:
Endophytic Mycobiota Characterization of the Amazonian Mistletoe Cladocolea micrantha Hosted in Cashew Tree
AUTHORS:
Anderson C. Guimarães, Antonio C. Siani, José L. Bezerra, Antonia Q. Lima de Souza, Maria Inez M. Sarquis
KEYWORDS:
Cladocolea micrantha; Endophytic mycobiota; Loranthaceae; Guignardia mangiferae ; Anacardium occidentale
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.4,
April
23,
2013
ABSTRACT: Endophytic
fungi were identified from
different parts of the medicinal parasitic mistletoe Cladocolea micrantha and from its host Anacardium occidentale, suggesting a strict host-parasite
relationship. Eight fungal endophytes were isolated and morphologically
characterized. The ascomycete Guignardia mangiferae and strains of Mycelia
sterilia were prevalent in the isolations. The unequivocal identification of Guignardia mangiferae at a
probabilistic degree close to 100% was
carried out by DNA extraction followed by PCR analyses of the ITS-1 and ITS-2 regions and
comparison of the genetic sequence with the NCBI database.