TITLE:
Esterases from the Seeds of an Edible Legume, Phaseolus vulgaris L.; Variability and Stability during Germination
AUTHORS:
Syed Azmal Ali, Grishma Bendre, Shraddha Ojha, V. Krishnamurthy, N. Ramachandrn Swarmy, Kempohalli S. Chandrashekharaiah
KEYWORDS:
Esterase; Phaseolus vulgaris; Germination; Variability; Stability
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.4,
April
23,
2013
ABSTRACT: The total protein and esterase were isolated from the
seeds of Red Kidney beans (Phaseolus
vulgaris). The crude protein content was observed as 15%. The
germination of seeds of red kidney bean has been carried out and change in the
total protein content and esterase activity was monitored. The protein content
was decreased from 15% (24 hours) to 8% (144 hours) during germination. The
socked seeds and seedlings of all the days of germination exhibited esterase activity.
Maximum ester hydrolyzing activity was observed on 6th day of germination whereas as lowest ester
hydrolyzing activity was observed 2nd day germination. Native PAGE was carried
out and esterase banding pattern for two different artificial substrates was
studied. The esterase banding pattern in presence of 1-Napthyl acetate showed
the presence of 4 esterolytic bands while 2 esterolytic bands were observed in
presence of 2-Napthyl acetate.