TITLE:
Long-term phenotypic characterization of human bone marrow and adipose tissue derived mesenchymal stromal cells
AUTHORS:
Dean L. Glettig, David L. Kaplan
KEYWORDS:
Stem Cells; Adipose Stem Cells; Mesenchymal Stem Cells; Flow Cytometry; Surface Markers; Human Cells; Long-Term Cell Culture
JOURNAL NAME:
Stem Cell Discovery,
Vol.3 No.2,
April
19,
2013
ABSTRACT: We
present methods to characterize mesenchymal stromal cells (MSC) over long time
periods in vitro. The methods entail
passaging cells multiple times and performing differentiation studies with the
cells at each passage. Using an array of surface markers and flow cytometric
quantification, the data can be correlated to traditional measures of
differentiation such as PCR and staining. Using these methods to quantify the
amount of differentiation, we concluded that many common MSC markers do not
specifically define MSCs with true stem cell properties. Additionally,
adipose-derived as opposed to bone marrow-derived MSCs show long-term CD34+ labeling. The methods described can be used to help identify stem cell
markers and to characterize the state of stem cells in vitro. Compiling these data from multiple laboratories would be
helpful to determine source, extraction and culture methods needed to obtain
high yields of useful stem cells.