TITLE:
Influence on proliferation and inducing capacity of bone mesenchymal stem cells during myocardial differentiation and construction of engineered myocardium-like tissue in vitro
AUTHORS:
Wanhong Xing, Lingping Tian, Jianhong Zhang, Ning Wang, Ping Chen, Jie Ma
KEYWORDS:
Mesenchymal Stem Cells; Proliferation; 5-Azacitidine; Cardiomyocytes; Myocardium; Tissue Engineering
JOURNAL NAME:
Stem Cell Discovery,
Vol.3 No.2,
April
19,
2013
ABSTRACT: The
study is to investigate BMSCs ability to differentiate cardiomyocytes,
especially discussed cell generations and 5-Aaz concentration influence on
BMSCs capability of proliferation and differentiation into cardiomyocytes
during constructing the engineered myocardium-like tissue in vitro. The results have demonstrated that the different
concentration of 5-Aza has the influence on proliferation rate of different generations
of BMSCs, the second generation BMSCs was superior to the sixth, and tenth
generation in proliferation capacity after being induced, and 5-Aza had some
influence on proliferation capacity. Rat BMSCs could be differentiated into
cardiomyocytes-like cells, which have a good biocompatibility with acellular
bovine pericardium, and myocardium-like tissue could be engineered with BMSCs
and acellular bovine pericardium in vitro.
In conclusion, BMSCs could be induced and differentiated into cardiomyocytes-like
cells in vitro. Different generations
and different 5-Aza density have influence on the rate of increase of BMSCs,
and the engineered myocardium-like tissue could be constructed with BMSCs and
acellular bovine pericardium in vitro.