TITLE:
Tyrosine phosphorylation of monocyte-derived macrophage proteins in buffalo (Bubalus bubalis): A potential phenotype of natural resistance
AUTHORS:
Maria Miarelli, Federica Signorelli, Giovanna de Matteis
KEYWORDS:
Buffalo; Macrophage; Natural Resistance; Tyrosine Phosphorylation
JOURNAL NAME:
Open Journal of Animal Sciences,
Vol.3 No.2,
April
18,
2013
ABSTRACT: The aim of this work was to explore the possibility of using the presence of
tyrosine-phosphorylated macrophage proteins as a phenotype of
natural resistance. Tyrosine-phosphorylation of macrophage proteins was
investigated in 18 buffaloes, that carried either the resistant, or the
non-resistant, Natural Resistance-Associated Macrophage Protein one (NRAMP1)
genotype, that various authors have associated with susceptibility to
intracellular bacterial diseases. Monocyte-derived macrophages were
Interferon-gamma (IFN-γ) stimulated and tyrosine-phosphorylation was
assessed by Western blotting. Evidence of phosphorylation after IFN-γ stimulation was shown by 75% of the
buffaloes carriers of the resistant genotype, and by 20% of the carriers of the
non-resistant genotype (Chisquare value between the groups = 5.44; P = 0.02).
The study of the Proteoma of monocyte-derived macrophages might open the way
to the genetic control of disease resistance.