TITLE:
Replicated association of the single nucleotide polymorphism in PNLIP with marbling in Niigata population of Japanese Black beef cattle
AUTHORS:
Hideki Tanomura, Youji Muramatsu, Takuji Yamamoto, Takeshi Ohta, Hiroyuki Kose, Takahisa Yamada
KEYWORDS:
Association; PNLIP; Japanese Black Breed; Marbling; Replication Study; Single Nucleotide Polymorphism
JOURNAL NAME:
Open Journal of Animal Sciences,
Vol.3 No.2,
April
18,
2013
ABSTRACT: Marbling
is regarded as an economically important trait of beef cattle inJapan, and measured
as a beef marbling score (BMS). Our previous study reported an association
between a single nucleotide polymorphism (SNP), rs4164 8172, in the pancreatic lipase (PNLIP) gene and the BMS level, using the Japanese Black beef cattle
population of Oita prefecture. Further, we showed that the T allele at the rs41648172
SNP is associated with a high level of the BMS. Thus, we suggested that the rs41648172 SNP seems to be a candidate
marker for marker-assisted selection. Our present study was designed to investigate
whether this association could be replicated in other independent Japanese
Black cattle population and analyze the effect of the SNP genotypes on the
carcass traits other than the BMS. We detected the marginally significant
effect of the genotypes of the rs41648172
SNP on the BMS level by using the Japanese Black beef cattle population of
Niigata prefecture (P = 0.0919), and obtained the result of the T allele associated with an increase in
the BMS level, consistent with our previous data. In addition, we showed no
significant association of the SNP with the subcutaneous fat thickness, carcass
weight, rib eye area, rib thickness and yield estimate in the Japanese Black
beef cattle population ofNiigataprefecture. Thus, we concluded that the rs41648172
SNP was useful for effective marker-assisted selection to increase the BMS
level in Japanese Black beef cattle, based on the replicated association of the rs41648172 SNP with the BMS level in
the other independent Japanese Black beef cattle population and no effect of
the SNP genotypes on the carcass traits other than BMS.