TITLE:
Detection of Influenza Viruses Attached to an Optical Disk
AUTHORS:
Takayuki Shima, Makoto Fujimaki, Akihiro Yoshida, Subash C. B. Gopinath, Masashi Kuwahara, Yoshimichi Ohki, Koichi Awazu
KEYWORDS:
Influenza Virus; Polystyrene Bead; Optical Disk; Laser; Reflectance; Sensor; Safety and Security
JOURNAL NAME:
Journal of Biomaterials and Nanobiotechnology,
Vol.4 No.2,
April
17,
2013
ABSTRACT: Detection of polystyrene beads with a diameter
of 100 nm was conducted using an optical disk drive tester equipped with a laser
of 405 nm wavelength and an objective lens with a numerical aperture of 0.65. The
polystyrene beads were used to mimic the influenza virus. A grooved disk with a
(ZnS)85(SiO2)15/SiO2 layered
surface structure was used for the detection. The detection of influenza
viruses (A/Udorn/307/1972) with Au nanoparticles was also demonstrated using
the optical disk drive tester. In this case, a grooved disk with an indium tin
oxide (ITO) film was used. The ITO film functioned both to tune the reflectance
of the disk and as an electrically conductive layer for scanning electron microscopy. In both cases, the target substances
were successfully recognized in a single scan with a high scanning speed of 4.9 m/s. The results indicate that this optical
disk system can be used to detect 100 nm scale substances like influenza
viruses, which are smaller than the diffraction limit of the system.