TITLE:
Thiocyanate Oxidation by Coculture from a Coke Wastewater Treatment Plant
AUTHORS:
Huijing Huang, Chunhua Feng, Xiaxia Pan, Haizhen Wu, Yuan Ren, Chaofei Wu, Chaohai Wei
KEYWORDS:
Thiocyanate Biodegradation; Carbon Source; Coke Wastewater; Biokinetics; Andrew-Haldane Model
JOURNAL NAME:
Journal of Biomaterials and Nanobiotechnology,
Vol.4 No.2A,
April
17,
2013
ABSTRACT: Bacterial strains in an activated sludge aerobic
reactor from a coke wastewater were found to be able to utilize thiocyanate as carbon source when the
thiocyanate-containing wastewater was deprived of carbon source. This study
showed that three thiocyanate-oxidizing bacterial strains, Burkholderia sp., Chryseobacterium sp., and Ralstonia sp. were isolated from the activated sludge of a
coke wastewater treatment plant as evidenced by the fact that complete decomposition of thiocyanate was achieved
either by coculture or individual pure culture. The thiocyanate biodegradation by
the coculture occurred with an optimal pH range between 6.5 and 8.5 and an
optimal temperature range between 30°C and 40°C. The biodegradation kinetics of thiocyanate was well fitted with the Andrew-Haldane model, which demonstrated a distinct substrate
concentration-inhibited bacterial growth pattern. The effects of different
types of additional carbon, nitrogen or sulfur sources on thiocyanate
biodegradation were also investigated. Analysis of the end-products indicated
that thiocyanate degradation by these strains should proceed via two pathways.