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L. Chen, K. Shiotani, T. Togashi, D. Miki, M. Aoyama, H. L. Wong, T. Kawasaki and K. Shimamoto, “Analysis of the Rac/Rop Small GTPase Family in Rice: Expression, Subcellular Localization and Role in Disease Resistance,” Plant and Cell Physiology, Vol. 51, 2010, pp. 585-595.

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• TITLE: Biotechnology and Plant Disease Control-Role of RNA Interference

  AUTHORS: Shabir H. Wani, Gulzar S. Sanghera, Naorem B. Singh

  KEYWORDS: RNAi, Viruses, Fungi, dsRNA, Gene Silencing

  JOURNAL NAME: American Journal of Plant Sciences, Vol.1 No.2, December 29, 2010

  ABSTRACT: Development of crop varieties which are resistant against many economically important diseases is a major challenge for plant biotechnologists worldwide. Although much progress in this area has been achieved through classical genetic approaches, this goal can be achieved in a more selective and robust manner with the success of genetic engineering techniques. In this regard, RNA interference (RNAi) has emerged as a powerful modality for battling some of the most notoriously challenging diseases caused by viruses, fungi and bacteria. RNAi is a mechanism for RNA-guided regulation of gene expression in which double-stranded ribonucleic acid (dsRNA) inhibits the expression of genes with complementary nucleotide sequences. The application of tissue-specific or inducible gene silencing in combination with the use of appropriate promoters to silence several genes simultaneously will result in protection of crops against destructive pathogens. RNAi application has resulted in successful control of many economically important diseases in plants.