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Konieczny, I. and Helinski, D.R. (1997) Helicase delivery and activation by DnaA and TrfA proteins during the initiation of replication of the broad host range plasmid RK2. Journal of Biological Chemistry, 272(52), 33312-33318.

has been cited by the following article:

• TITLE: Cloning, expression, purification and characterization of replication protein from plasmid pGP2 from Acetobacter estunensis

  AUTHORS: Peter Grones, Jozef Grones

  KEYWORDS: Acetobacter Estunensis; Rep34 Protein; DNA-Binding Activity; ATPase Activity; Phosphatase Activity; Unwinding Activity

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.1 No.5, December 30, 2010

  ABSTRACT: The Acetobacter estunensis Rep34 protein participates in the replication of bacterial plasmid pGP2. The Rep34 protein of the A. estunensis, was cloned to the expression vector, that ensure fusion with a His-tag sequence (Rep34 His-tagged), over-expressed in Escherichia coli and purified by metal-affinity chromatography to yield a highly purified and active protein. On this purified protein number different activities and motifs were detected. DNA band-shift assays showed that the Rep34 His-tagged protein bound to the regulation region for replication on the linear double-stranded DNA. In the protein was determined phosphatase activity, ATPase activity and protein is possible to unwind double strand DNA.