TITLE:
Isolation and Identification of Two Antibacterial Agents from Chromolaena odorata L. Active against Four Diarrheal Strains
AUTHORS:
Ménonvè Atindehou, Latifou Lagnika, Bernard Guérold, Jean Marc Strub, Minjie Zhao, Alain Van Dorsselaer, Eric Marchioni, Gilles Prévost, Youssef Haikel, Corinne Taddéi, Ambaliou Sanni, Marie-Hélène Metz-Boutigue
KEYWORDS:
Chromolaena odorata; 3’, 4’, 5, 6, 7-Pentamethoxyflavone (Sinensetin); 4’, 5, 6, 7-Tetramethoxyflavone (Scutellareintetramethyl ether); Antibacterial Activity; Diarrheal Strains
JOURNAL NAME:
Advances in Microbiology,
Vol.3 No.1,
March
29,
2013
ABSTRACT: Chromolaena odorata L (Asteraceae) is a bad invasive plant, found in the humid tropics and sub-tropics worldwide. It is used against dysentery, diarrhea, malaria, wound healing, headache and toothache in traditional medicine. In the present study, we investigated the antibacterial activities of different leaves extracts of Chromolaena odorata L. (cyclohexane, dichloromethane, ethyl acetate and butanol) against four clinical diarrheal strains (Klebsiella oxytoca, Salmonella enterica, Shigella sonnei and Vibrio cholera). We demonstrated that C. odorata leaves extracts show an antibacterial activity between 0.156 and 1.25 mg/mL. Bioassay-guided chromatography by bioautography with iodonitrotetrazolium-based colorimetric assay allowed the isolation and identification of two active compounds. After the combination of RP-HPLC, mass spectrometry analysis, 1D and 2D-NMR spectroscopy, we isolated and characterized two active molecules corresponding to 3’,4’,5,6,7-Pentamethoxyflavone (Sinensetin) and4’,5,6,7-Tetramethoxyflavone (Scutellareintetramethyl ether).