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Ohnishi, M., Tanaka, C., Kuhara, S., Ishii, K., Hattori, M., Kurokawa, K., Yasunaga, T., Makino, K., Shinagawa, H., Murata, T., Nakayama, K., Terawaki, Y. and Hayashi, T. (1999) Chromosome of the enterohemorrhagic Escherichia coli O157:H7; comparative analysis with K-12 MG 1655 revealed the acquisition of a large amount of foreign DNAs. DNA Research, 6, 361-368. doi:10.1093/dnares/6.6.361

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• TITLE: Metabolic regulation of Escherichia coli cultivated under anaerobic and aerobic conditions in response to the specific pathway gene knockouts

  AUTHORS: Yu Matsuoka, Kazuyuki Shimizu

  KEYWORDS: Metabolic Regulation; Single-Gene Knockout Mutant; Escherichia coli

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.4 No.3A, March 29, 2013

  ABSTRACT: Effect of the specific gene knockout on the main metabolism in Escherichia coli was reviewed, and the regulation mechanisms were clarified based on different levels of information such as gene expressions, enzyme activities, intracellular metabolite concentrations, and metabolic fluxes together with fermentation data. The effects of the knockout of such genes as pflA, pta, ppc, pykF, adhE, and ldhA on the metabolic changes were analyzed for the case under anaerobic condition. The effects of the knockout of such genes as pgi, zwf, gnd, ppc pck, pyk, and lpdA on the metabolic changes were also analyzed for the case under aerobic condition. The metabolic regulation analysis was made focusing on the roles of transcription factors.