TITLE:
PCR-DGGE analysis of earthworm gut bacteria diversity in stress of Escherichia coli O157:H7
AUTHORS:
Yi Zhang, Gaochan Wang, Yupeng Wu, Hui Zhao, Yufeng Zhang, Zhenjun Sun
KEYWORDS:
PCR-DGGE; Earthworm Intestinal Bacteria Diversity; Escherichia coli O157:H7 Stress
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.3A,
March
29,
2013
ABSTRACT: In order to test if the intestinal bacteria play an
important role in antibacterial ability of earthworm, we chose Escherichia
coli O157:H7, an
anthropozoonosis pathogen, as a biological stressor and studied the change of intestinal bacteria community of earthworm by
PCR-DGGE analysis. Results showed that the pathogen merely existed 1 - 3 days,
then almost disappeared after through the earthworm’s gut. In this period, the
diversity and abundance index of intestinal bacteria increased first, then
decreased, and finally kept stably after 7
days. The result demonstrated that the intestinal bacteria of earthworm
had ability of adjust community structure to eliminate the pathogen E. coli O157:H7, and the amount of bacteria Bacillus increased significantly,
which might be the positive antagonism to E. coli O157:H7.