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Liu, X.L., Zhang, N., Cui, D.L., Wang, C. and Sun, Z.J. (2011) Lactic acid bacteria isolated from vermicompost and its antimicrobial activity in E. coli O157:H7. GAB, 1, 1-6

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• TITLE: PCR-DGGE analysis of earthworm gut bacteria diversity in stress of Escherichia coli O157:H7

  AUTHORS: Yi Zhang, Gaochan Wang, Yupeng Wu, Hui Zhao, Yufeng Zhang, Zhenjun Sun

  KEYWORDS: PCR-DGGE; Earthworm Intestinal Bacteria Diversity; Escherichia coli O157:H7 Stress

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.4 No.3A, March 29, 2013

  ABSTRACT: In order to test if the intestinal bacteria play an important role in antibacterial ability of earthworm, we chose Escherichia coli O157:H7, an anthropozoonosis pathogen, as a biological stressor and studied the change of intestinal bacteria community of earthworm by PCR-DGGE analysis. Results showed that the pathogen merely existed 1 - 3 days, then almost disappeared after through the earthworm’s gut. In this period, the diversity and abundance index of intestinal bacteria increased first, then decreased, and finally kept stably after 7 days. The result demonstrated that the intestinal bacteria of earthworm had ability of adjust community structure to eliminate the pathogen E. coli O157:H7, and the amount of bacteria Bacillus increased significantly, which might be the positive antagonism to E. coli O157:H7.