TITLE:
Effect of Hg(II) and Pb(II) Ions on C-Phycocyanin (Spirulina platensis)
AUTHORS:
Eteri Gelagutashvili, Ketevan Tsakadze
KEYWORDS:
C-Phycocyanin; Hg(II); Pb(II) Ions; DNA; Fluorescence
JOURNAL NAME:
Optics and Photonics Journal,
Vol.3 No.1,
March
29,
2013
ABSTRACT: Influence of Hg(II) and Pb(II) ions on C-Phycocyanin (C-PC) from cyanobacteria Spirulina platensis was investigated using Fluorescence spectroscopy. Fluorescence measurements demonstrate quenching of C-PC emission by Hg(II) and Pb(II), and blue shifts in the fluorescence spectra. The effect of DNA on the fluorescence of Hg(II)-and Pb(II)-C-PC (from Spirulina platensis) complexes was also studied. It was shown that the fluorescence intensity of Hg-C-PC after addition of DNA gave rise to the fluorescence buildup. At the same time, addition of DNA to the Pb(II)-C-PC complexes showed no such effect. In the case of Hg(II)-C-PC, fluorescence intensity significantly decreases in time, while for Pb(II)-C-PC, decrease of the fluorescence intensity is not significant, but blue shift of the peak takes place.