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Simkin, A. J., Moreau, H., Kuntz, M., Pagny, G., Chen-wei, L., Tanksley, Se., and McCarthy, J. (2008) An inves-tigation of carotenoid biosynthesis in Coffea canephora and Coffea Arabica. Journal of Plant Physiology, 165(10), 1087-1106.

has been cited by the following article:

• TITLE: Optimization of solvent extraction conditions for total carotenoids in rapeseed using response surface methodology

  AUTHORS: Ling Wang, Yun Liu

  KEYWORDS: Carotenoids; Optimization; Solvent Extraction; Response Surface Methodology; Rapeseed

  JOURNAL NAME: Natural Science, Vol.1 No.1, July 2, 2009

  ABSTRACT: The optimum total carotenoids (TC) extraction from rapeseed with solvent extraction method by UV-visible spectrophotometer determination was investigated by using response surface methodology (RSM). Extraction duration, re-peated extraction cycles, solvent-solid ratio and extraction temperature were assumed to be the most important factors affecting solvent extrac-tion for the determination of TC. Optimum sol-vent extraction conditions for maximizing the determination of TC were: extraction duration 7.3h, repeated extraction three times, ratio of solvent-solid (v/w, mL/mg) 29:1, extraction temperature 42°C. Under the optimal conditions, the yield of TC was up to 4.79 mg /100g. The model had a satisfactory coefficient of R2 (= 0.912) and verified experimentally. The results showed that the conditions were mild and use-ful for maximizing a quantitative spectropho-tometer determination of TC in rapeseed.