TITLE:
Growth Yields of Four Coxiella burnetii Isolates in Four Different Cell Culture Lines
AUTHORS:
Michelle G. Lockhart, Aminul Islam, Stanley G. Fenwick, Stephen R. Graves, John Stenos
KEYWORDS:
Bacterial Yield; Cell Culture; Coxiella burnetii; Intracellular Bacteria
JOURNAL NAME:
Advances in Microbiology,
Vol.3 No.1,
March
28,
2013
ABSTRACT: Although Coxiella burnetii is considered to be an obligate intracellular
bacterium and grows in embryonated eggs, laboratory animals and cell culture,
recently it has been grown in cell-free media and on agar plates. This current
study was conducted to compare four cell lines for their yield of C. burnetii. Four different isolates of C. burnetii (Henzerling, Arandale, Cumberland and Timony) were grown in DH82, L929, Vero
and XTC-2 cell lines. The DH82 and
XTC-2 cells lines produced the highest C. burnetii yield which was slightly less than
the yields achieved in recently published studies using cell free media. The
Arandale isolate of C. burnetii produced
a significantly higher yield in DH82 cells compared to XTC-2 cells (P 0.03).