TITLE:
Optimization of solvent extraction conditions for total carotenoids in rapeseed using response surface methodology
AUTHORS:
Ling Wang, Yun Liu
KEYWORDS:
Carotenoids; Optimization; Solvent Extraction; Response Surface Methodology; Rapeseed
JOURNAL NAME:
Natural Science,
Vol.1 No.1,
July
2,
2009
ABSTRACT: The optimum total carotenoids (TC) extraction from rapeseed with solvent extraction method by UV-visible spectrophotometer determination was investigated by using response surface methodology (RSM). Extraction duration, re-peated extraction cycles, solvent-solid ratio and extraction temperature were assumed to be the most important factors affecting solvent extrac-tion for the determination of TC. Optimum sol-vent extraction conditions for maximizing the determination of TC were: extraction duration 7.3h, repeated extraction three times, ratio of solvent-solid (v/w, mL/mg) 29:1, extraction temperature 42°C. Under the optimal conditions, the yield of TC was up to 4.79 mg /100g. The model had a satisfactory coefficient of R2 (= 0.912) and verified experimentally. The results showed that the conditions were mild and use-ful for maximizing a quantitative spectropho-tometer determination of TC in rapeseed.