TITLE:
Association between Plasma Myeloperoxidase and Free 3-Nitrotyrosine Levels in Patients with Coronary Artery Disease
AUTHORS:
Morteza Pourfarzam, Ahmad Movahedian, Nizal Sarrafzadegan, Gholam Basati, Saed Ziaaldin Samsamshariat
KEYWORDS:
Myeloperoxidase; 3-Nitrotyrosine; Stable; Unstable CAD
JOURNAL NAME:
International Journal of Clinical Medicine,
Vol.4 No.3,
March
28,
2013
ABSTRACT: Objective: Myeloperoxidase (MPO) is an inflammatory enzyme that is
mainly released by activated neutrophils and monocytes. 3-nitrotyrosine (NT) is
a stable inflammatory end product of MPO that is produced through nitrosylation
of free and protein-bound tyrosines. Determination of the exact levels of free
NT is technically a challenging matter. Also, there is limited information
about the relationship between MPO and free NT levels and elevation of them in
the plasma of patients with coronary artery disease (CAD). Therefore, we sought
to determine the exact level of plasma free NT with a simple and exquisite
technique in CAD patients. Methods: This study included 50 stable
angina, 50 unstable angina patients, and 50 control subjects. Plasma MPO concentration was
measured with an immunoassay method. Plasma free NT level was determined by a
modified HPLC-fluorescence method. Lipid profile, high sensitivity C-reactive protein (hsCRP) and other
clinical risk factors of patients were also assigned. Results: Plasma
level of free NT was efficiently
measured by the HPLC-fluorescence
method. Plasma levels of MPO and NT were significantly higher in patients with
stable and unstable CAD than in control subjects (P Conclusions: We determined plasma free NT levels with a
sensitive HPLC-fluorescence method with some modifications in a clinical scale.
Plasma levels of MPO and NT were profoundly elevated in CAD patients. The significant
relationships of the two substances and elevation of them may have useful
clinical implication in patients with stable and unstable CAD.