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V. Mai, W. W. Lorenz and J. Wiegel, “Transformation of Thermoanaerobacterium sp. Strain JW/SL-YS485 with Plasmid pIKM1 Conferring Kanamycin Resistance,” FEMS Microbiology Letters, Vol. 148, No. 2, 1997, pp. 163-167. doi:10.1111/j.1574-6968.1997.tb10283.x

has been cited by the following article:

• TITLE: Metabolic Engineering of Thermoanaerobacterium thermosaccharolyticum for Increased n-Butanol Production

  AUTHORS: Ashwini Bhandiwad, Anna Guseva, Lee Lynd

  KEYWORDS: Biofuels; n-Butanol; Thermophile; Metabolic Engineering

  JOURNAL NAME: Advances in Microbiology, Vol.3 No.1, March 27, 2013

  ABSTRACT: Thermoanaerobacterium thermosaccharolyticum shows promise as a host for n-butanol production since it natively has the required genes involved in the n-butanol biosynthetic pathway. Overexpression of the natively occurring bcs operon containing the genes thl, hbd, crt, bcd, etfA, and etfB responsible for the formation of butyryl CoA increased the n-butanol production by 180% compared to the wild type from a n-butanol titer of 1.8 mM to 5.1 mM. The deletion of one of the six alcohol dehydrogenase genes confirmed that it was the primary gene responsible for ethanol and n-butanol production from acetyl CoA and butyryl CoA respectively.