TITLE:
Activation of Ethanol Production by Combination of Recombinant Ralstonia eutropha and Electrochemical Reducing Power
AUTHORS:
Bo Young Jeon, Jun Yeong Yi, Il Lae Jung, Doo Hyun Park
KEYWORDS:
Ralstonia eutropha; Electrochemical Reducing Power; Genetic Recombination; Pyruvate Decarboxylase; Alcohol Dehydrogenase
JOURNAL NAME:
Advances in Microbiology,
Vol.3 No.1,
March
27,
2013
ABSTRACT: Ralstonia eutropha was genetically modified to induce ethanol
production from glucose. An electrochemical bioreactor was prepared to generate
electrochemical reducing power coupled to regeneration of NADH. Growing cells
of recombinant R. eutropha produced
about 29 mM of ethanol in conventional conditions and 56 mM of ethanol in electrochemically
reduced conditions from 100 mM glucose. Grown cells of the recombinant produced
about 52 mM of ethanol in conventional conditions and 142 mM of ethanol in
electrochemically reduced condition from 100 mM glucose. These results are a clue
that electrochemical reducing power can induce the recombinant R. eutropha to produce more ethanol
coupled to increase of NADH/NAD+ ratio.