TITLE:
Pre-Enrichment of Estuarine and Fresh Water Environmental Samples with Sodium Chloride Yields in Better Recovery of Vibrio parahaemolyticus
AUTHORS:
Yoshimitsu Otomo, Farzana Hossain, Fazle Rabbi, Yuki Yakuwa, Chowdhury Rafiqul Ahsan
KEYWORDS:
Vibrio parahaemolyticus; Sodium Chloride; Estuary; Fresh Water
JOURNAL NAME:
Advances in Microbiology,
Vol.3 No.1,
March
27,
2013
ABSTRACT: Vibrio
parahaemolyticus organisms cause acute
gastroenteritis in humans. These bacteria are natural inhabitants of both
marine and estuarine ecosystems. In the present study, we investigated the
effectiveness of a non-selective enrichment of sediment samples with sodium
chloride prior to selective enrichment with alkaline peptone water for a better
recovery of V. parahaemolyticus.
Sediment samples were collected with or without 1% NaCl from the river
Buriganga, located besides Dhaka city and about 400 km away from the Bay of
Bengal, and from the estuary of the river Karnaphuli which flows into the Bay
of Bengal. Very small number of V.
parahaemolyticus (V. parahaemolyticus increased to more
than 40 times (1500 MPN/g) in the river and 32 times (960 MPN/g) in the estuary
where NaCl were added. River sediment sample contained the serotype O9:K41 of V. parahaemolyticus and the estuarine
sample contained O3:K41 and O3:KUT Our results suggest that a pre-enrichment
of environmental samples with 1% NaCl helps V.
parahaemolyticus to survive for at least 7 days until they are enriched
with alkaline peptone water, for better recovery.