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J. Tada, N. Ohashi, N. Nishimura, Y. Shirasaki, H. Ozaki, S. Fukushima, J. Takano, M. Nishibuchi and Y. Takeda, “Detection of the Thermostable Direct Hemolysin Gene (tdh) and the Thermostable Direct Hemolysin-Related Hemolysin Gene (trh) of Vibrio parahaemolyticus by Polymerase Chain Reaction,” Molecular and Cellular Probes, Vol. 6, No. 6, 1992, pp. 477-487. doi:10.1016/0890-8508(92)90044-X

has been cited by the following article:

• TITLE: Pre-Enrichment of Estuarine and Fresh Water Environmental Samples with Sodium Chloride Yields in Better Recovery of Vibrio parahaemolyticus

  AUTHORS: Yoshimitsu Otomo, Farzana Hossain, Fazle Rabbi, Yuki Yakuwa, Chowdhury Rafiqul Ahsan

  KEYWORDS: Vibrio parahaemolyticus; Sodium Chloride; Estuary; Fresh Water

  JOURNAL NAME: Advances in Microbiology, Vol.3 No.1, March 27, 2013

  ABSTRACT: Vibrio parahaemolyticus organisms cause acute gastroenteritis in humans. These bacteria are natural inhabitants of both marine and estuarine ecosystems. In the present study, we investigated the effectiveness of a non-selective enrichment of sediment samples with sodium chloride prior to selective enrichment with alkaline peptone water for a better recovery of V. parahaemolyticus. Sediment samples were collected with or without 1% NaCl from the river Buriganga, located besides Dhaka city and about 400 km away from the Bay of Bengal, and from the estuary of the river Karnaphuli which flows into the Bay of Bengal. Very small number of V. parahaemolyticus (V. parahaemolyticus increased to more than 40 times (1500 MPN/g) in the river and 32 times (960 MPN/g) in the estuary where NaCl were added. River sediment sample contained the serotype O9:K41 of V. parahaemolyticus and the estuarine sample contained O3:K41 and O3:KUT Our results suggest that a pre-enrichment of environmental samples with 1% NaCl helps V. parahaemolyticus to survive for at least 7 days until they are enriched with alkaline peptone water, for better recovery.