TITLE:
Diversity and Bioactivity of Cultivable Animal Fecal Actinobacteria
AUTHORS:
Yi Jiang, Li Han, Xiu Chen, Min Yin, Dan Zheng, Yong Wang, Shumei Qiu, Xueshi Huang
KEYWORDS:
Actinobacteria; Diversity; Bioactivity; Animal Feces
JOURNAL NAME:
Advances in Microbiology,
Vol.3 No.1,
March
27,
2013
ABSTRACT: Microbial symbionts play important roles in food digestion and absorption,
immunity, pathogens resistance, and health maintaining of their hosts by
coevolution. To provide new sources for discovering new leader compounds of
drugs, the diversity and bioactivities of cultivable actinobacteria from animal
feces have been studied. 31 species of animal fecal samples were collected fromYunnanWildAnimalPark. The purified
cultures of actinobacteria were isolated from these samples by using 5 media.
The 16S rRNA gene sequences of 528 selected strains were determined, the phylogenetic analysis was carried out, and
anti-microbial and anti-tumor activities were determined. 35 genera (including
a new genus, Enteractinococcus) of actinobacteria from the 31 species of animal
feces were identified. Some strains had high anti-tumor and antimicrobial
activities. More than 50 secondary metabolites were isolated and identified, a
novel bioactive macrolactam polyketide glycoside, Sannastatin, was found. Nine
fecal streptomycete strains were fermented respectively, blended to the
microbial manure, and used to prophylaxis and treatment of soil-borne disease
of notoginseng in
field. The incidence rate of the disease was lower 81% than agricultural
chemicals. Fecal actinobacteria, a possibility as a new source for discovering
drug leader, agricultural chemicals and other industry products, will be
discussed.