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K. Takahashi, R. S. Goomer, F. Harwood, T. Kubo, Y. Hirasawa and D. Amiel, “The Effects of Hyaluronan on Matrix Metalloproteinase-3 (MMP-3), Interleukin-1Beta (IL-1Beta), and Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) Gene Expression during the Development of Osteoarthritis,” Osteoarthritis Cartilage, Vol. 7, No. 2, 1999, pp. 182-190. doi:10.1053/joca.1998.0207

has been cited by the following article:

• TITLE: Hyaluronate Increases Polynucleotides Effect on Human Cultured Fibroblasts

  AUTHORS: Stefano Guizzardi, Jacopo Uggeri, Silvana Belletti, Giulia Cattarini

  KEYWORDS: Polynucleotides; Fibroblasts, Hyaluronate; Dermal Regeneration

  JOURNAL NAME: Journal of Cosmetics, Dermatological Sciences and Applications, Vol.3 No.1, March 27, 2013

  ABSTRACT: The HA is present in almost all vertebrates and plays a critical role in tissue development and cell proliferation, it has been demonstrated to promote wound healing and involved in angiogenesis and inflammation. Also polynucleotydes (PN) have proved to promote the “in vitro” growth and activity of human fibroblasts and osteoblasts, to increase reparation on UVB damaged dermal fibroblasts and seems to promote proliferation of human pre-adipocytes. Several in vivo studies have demonstrated the PN effect also in vivo, inducing an increase of angiogenesis and healing process. In this paper we have evaluated the effect of a mixture of Polynucleotides (PN) and entire Hyaluronic Acid (HA) on cultured human fibroblasts by analyzing cell growth. Different mixture have been tested and it has been demonstrated that the presence of HA even at low concentration (1 mg/ml) determine an increase of PN activity up to 20%. Furthermore, the addition of HA 1 mg/ml to PN 100 μg/ml induces a cell growth rate comparable to that exerted by PN concentration of 12 μg/ml.