TITLE:
Overexpression of ARAhPR10, a Member of the PR10 Family, Decreases Levels of Aspergillus flavus Infection in Peanut Seeds
AUTHORS:
Chunzheng Xie, Shijie Wen, Haiyan Liu, Xiaoping Chen, Haifen Li, Yanbin Hong, Xuanqiang Liang
KEYWORDS:
Peanut; PR10; Aflatoxin Contamination; Overexpression
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.3,
March
26,
2013
ABSTRACT: Peanut (Arachis hypogaea L.) is one of the most susceptible host crops to Aspergillus flavus invasion and subsequent aflatoxin contamination. In this report, a new member of PR10 family putative resistant gene (designated as ARAhPR10, No. EU661964.1) encoding a PR10 protein was isolated and characterized. Analysis of qRT-PCR showed that the expression of ARAhPR10 was induced by pre-harvested A. flavus infection, but no significant difference was observed between resistant genotype “GT-C20” and susceptible genotype “Yueyou 7”. Seven transgenic peanut lines expressing the ARAhPR10 gene under the control of 35S promoter were obtained using the Agrobacterium tumefaciens-mediated method. Real time RT-PCR results showed that the expression level of the ARAhPR10 was significantly higher and the A. flavus infection and aflatoxin content were significantly lower in seeds of transgenic lines than that of the wild type. A significant negative correlation between ARAhPR10 expression at transcript level and seeds aflatoxin production was observed. Combining the previous results, it is suggested that ARAhPR10 expression play an important role in peanut host resistance to A. flavus infection and aflatoxin producing.