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Weerkamp, A.H., Quirynen, M., Marechal, M., Van der Mei, H.C., Van Steenberghe, D. and Busscher, H. J. (1989) The role of surface free energy in vivo formation of dental plaque on human enamel and polymeric substrata. Microbial Ecology in Health and Disease, 2, 11-18. doi:10.3109/08910608909140196

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• TITLE: Effect of zeta potentials on bovine serum albumin adsorption on crown composite resin surfaces in vitro

  AUTHORS: Nahoko Miyake, Tadashi Miura, Toru Sato, Masao Yoshinari

  KEYWORDS: Zeta Potential; Crown Composite Resins; Bovine Serum Albumin; Adsorption; Electrostatic Interactions

  JOURNAL NAME: Journal of Biomedical Science and Engineering, Vol.6 No.3, March 21, 2013

  ABSTRACT: We previously studied the mechanism underlying the adsorption of oral bacteria on the surfaces of dental prosthetic materials such as ceramics and resins in vitro. The aim of the present study was to examine bovine serum albumin (BSA) adsorption on crown composite resin surfaces by means of zeta potential. We measured the zeta potentials of resins alone, BSA alone, and resins after BSA adsorption. Eight resins were pulverized into powders (300 - 1000 nm). All experiments were conducted in 10 mM sodium chloride solution (pH 6.5). BSA was dissolved in 10 mM NaCl with a concentration of 2.0 × 10-5 mol/l. An adsorption assay was performed for one hour at 37°C under continuous rotation (6 rpm). The zeta potentials of both resins and BSA were negative, with BSA itself less negative than the resins themselves as an absolute value (p