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Siwoski, A., Ishkanian, A., Garnis, C., Zhang, L., Rosin, M. and Lam, W.L. (2002) An efficient method for the assessment of DNA quality of archival microdissected specimens. Modern Pathology, 15, 889-892. doi:10.1097/01.MP.0000024288.63070.4F

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• TITLE: Senescence process and oxidative stresses induce changes in plant genomic DNA quality

  AUTHORS: Sedigheh Hatami-Gigloo, S. M. Mahdi Mortazavian, Mojdeh Hatami-Gigloo, Masoud Ghorbani

  KEYWORDS: RAPD; Senescence; Oxidative Stress; Genetically Erosion

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.4 No.3, March 20, 2013

  ABSTRACT: Senescence or programmed cell death is a process that interacts with many biochemical and physiological changes in living organism and is generally induced by aging. Many environmental stresses that accelerate the production of activated oxygen can also induce senescence artificially. One of the important aspects of senescence is possibly degradation of macromolecules such as DNA. It is believed that the random amplification of polymorphic DNA (RAPD) technique is a good method to compare the DNA quality of juvenile and senescence samples in which oxidative stress is induced. In this study, juvenile, senescence and plant paraquat treated leaves from tomato, tobacco and rose, as well as juvenile and senescence human tissues were processed for DNA extraction followed by RAPD technique. We discovered that plant and human genomes are influenced by senescence and environmental stresses underwent genome diversity. Using some molecular markers proved that senescence and oxidative treated samples show different DNA pattern compare to the juvenile-un- treated samples. We also concluded that RAPD technique can be used as a useful tool in genomics study to provide researchers reliable information of DNA quality and can effectively help to resolve the environment condition.