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M. Ohira, F. Miyahara, Y. Mori, M. Okawa, J. Miyazaki, S. Masaki, K. Yoshimoto, Y. Sasaki, Y. Yamada, Y. Mitsugi, T. Tagami, T. Koyama, M. Miyazato, M. Tobase, K. Kuroda, M. Okamura, K. Matsunaga and S. Shiraishi, “Development of a Novel Cutting-Production System for Second-Generation Pinus thunbergii Resistant to Pine Wilt Disease,” Forest Tree Breeding, Vol. 235, 2010, pp. 1-5.

has been cited by the following article:

• TITLE: Metabolites from Resistant and Susceptible Pinus thunbergii after Inoculation with Pine Wood Nematode

  AUTHORS: Fujia Zhang, Junko Kajiwara, Yasuhiro Mori, Mineko Ohira, Yuji Tsutsumi, Ryuichiro Kondo

  KEYWORDS: Benzoic Acid; Bursaphelenchus xylophilus; Linoleic Acid; Pinosylvin; Pinus thunbergii; Resistance

  JOURNAL NAME: American Journal of Plant Sciences, Vol.4 No.3, March 15, 2013

  ABSTRACT: Pine wilt disease (PWD), which is caused by pine wood nematodes (PWN), is one of the most serious forest diseases worldwide. To clarify the mechanism of resistance to PWD, we compared metabolites from resistant and susceptible Japanese black pine (Pinus thunbergii) families after inoculation with PWN. After 2 weeks to 1 month post inoculation, the number of PWN dramatically increased in susceptible plants, but not in resistant plants. At this PWN-proliferation phase, ethyl acetate soluble fractions extracted from PWN-inoculated plants were analyzed by gas chromatogramphy-mass spectrometry (GC-MS). Although most compounds were qualitatively and quantitatively similar between resistant and susceptible plants, resistant plants accumulated 2.0-fold more linoleic acid (LA) than susceptible plants. On the other hand, benzoic acid (BA) was barely detected in resistant plants, but it accumulated in susceptible plants as the number of PWN increased. Susceptible plants contained greater levels of the nematicidal compounds pinosylvin and pinosylvin monomethyl ether, compared with resistant plants. These results suggested that LA is involved in the resistance reaction against PWN-proliferation, and that BA could be a good biomarker for PWD.