TITLE:
Age changes of antyoxidant system and free-radical processes in rats at formation of postinfarction cardiosclerosis
AUTHORS:
Tatiana Rebrova, Sergey Afanasiev, Olesia Putrova, Roman Batalov, Sergey Popov
KEYWORDS:
Lipid Peroxidation; Nitrogen Oxide; Postinfarction Cardiosclerosis; Age-Related Aspect
JOURNAL NAME:
Advances in Aging Research,
Vol.2 No.1,
February
28,
2013
ABSTRACT: Estimation of age dependent changes of the lipid
peroxidation (LPO) intensity, content of stable metabolites of nitrogen oxides
(NO) and antioxidant enzyme activities in rat blood serum in conditions of
experimental postinfarction cardiosclerosis (PICS) is carried out. Initiation
of the postinfarction remodeling of animals has been carried out with coronary
occlusion, definition of LPO and NO metabolites indices has been performed
after 45 days. Investigations have been carried out on 40 four and twelve-month-old
male Wistar rats with mass 200-250 gand 400
-450 g, accordingly. Statistical
analysis of the results was performed using the Mann-Whitney-Wilcoxone
criterion. It has been found that already intact animals have age specificity
of indices under consideration. The expressed activity of LPO processes on the
background of reduction of endogenous fermentative antioxidant (SOD and
catalase) activity as well as nitrite concentration in blood serum is
characteristic for PICS of 4-month-old animals. PICS of 12 month-old rats is
accompanied with suppression of the LPO processes on the back- ground of
reduction of the antioxidant enzyme intensity and increase of NO metabolites
production. The following conclusions have been drawn. Process of ontogenesis
is characterized by imbalance between pro-and antioxidant processes in rat
blood. Increase in catalase activity and concentration of the TBC-active
products at simultaneous decrease of SOD activity and content of diene
conjugates has been noted. The organism of young animals responds with persistent
increase of LPO processes and decrease of SOD and catalase activity on formation of postinfartion
cardioslerosis. The less expressed increase of lipid peroxidation activation
and decrease of
catalase activity has been noted in the organism of old animals within 45 day
after PICS formation.