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M. H. Frick, O. Elo, H. Kauko, O. P. Heinonen, P. Heinsalmi, P. Helo, J. K. Huttunen, P. Kaitaniemi, P. Koskinen, V. Manninen, H. M?enp??, M. M?lk?nen, M. M?ntt?ri, S. Norola, A. Pasternack, J. Pikkarainen, M. Romo, T. A. Sj?blom and E. A. Nikkil?, “Helsinki Heart Study: Primary-Prevention Trial with Gemfibrozil in MiddleAged Men with Dyslipidemia,” The New England Journal of Medicine, Vol. 317, No. 20, 1987, pp. 1237-1245. doi:10.1056/NEJM198711123172001

has been cited by the following article:

• TITLE: Effect of Dai-Saiko-To (Da-Chai-Hu-Tang) on LDL-Receptor Gene Expression in Human Hepatoma Cell Line (HepG2)

  AUTHORS: Akira Iizuka, Fumihiko Yoshie, Sakae Amagaya, Takaaki Yasuda, Maki Iizuka, Haruyo Yamaguchi, Seiji Nagumo, Kazuo Kondo

  KEYWORDS: Kampo; Dai-Saiko-To; LDL Receptor; Gene Expression; HepG2

  JOURNAL NAME: American Journal of Plant Sciences, Vol.4 No.2A, February 28, 2013

  ABSTRACT: We previously reported that Dai-saiko-to (Da-Chai-Hu-Tang), a traditional Japanese kampo medicine, increased LDL receptor mRNA expression in the liver of the hypercholesterolemic rabbits. In this study, we focused on LDL receptor gene expression in a human hepatoma cell line (HepG2) treated with Dai-saiko-to extract and the extracts of eight herbs presented in Dai-saiko-to. Dai-saiko-to extract significantly increased LDL receptor gene and SREBP2 gene expression compared with the control. The extracts of four herbs, Bupleurum root, Pinellia tuber, Scutellaria root and Peony root significantly increased the LDL receptor gene expression. Whereas, Jujube, Immature orange, Ginger and Rhubarb extracts did not change the gene expression. These results suggest that Dai-saiko-to increased the expression of the cholesterol transport gene (LDL receptor) regulated by SREBP2 gene in the human hepatoma cell line. The pharmacological activity of Dai-saiko-to against hypercholesterolemia and atheromatous lesions related for these four herbal components.