TITLE:
Rapid In-Vitro and In-Vitro Detection of Chalara fraxinea by Means of Mass Spectrometric Techniques
AUTHORS:
Thi Lam Huong Pham, Irmtraut Zaspel, Michael Schuemann, Heike Stephanowitz, Eberhard Krause
KEYWORDS:
Chalara fraxinea; Rapid In-Vitro; In-Vitro Detection; MS Techniques; MALDI; LC-MS
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.2A,
February
28,
2013
ABSTRACT: For
the first time, mass spectrometric (MS) techniques were employed to rapidly detect
the pathogen Chalara fraxinea in-vitro and directly in-vivo in tissues of diseased ash trees caused by C. fraxinea, using a range of characteristic
novel secondary metabolites of C. fraxinea as chemical markers for the presence
of the pathogen. We have found an evident
correlation between the presence and amount of these-only
for C. fraxinea characteristic and novel-secondary metabolites (named
chalarafraxinines) and the degree of disease
of respective infected ash seedlings. As demonstrated in this work, the MS
based high-throughput-screening approach
constitute an alternative to the time consuming and expensive micro biological isolation procedures for detection of the
pathogen C. fraxinea and furthermore, can be used to rapidly test ash genotypes
for resistance / susceptibility to C. fraxinea infection.