TITLE:
Functional Analysis of a Wilt Fungus Inducible PR10-1 Gene from Cotton
AUTHORS:
Lijun Chen, Na Sun, Jin Wang, Hua Ling, Lida Zhang, Kaijing Zuo
KEYWORDS:
Pathogenesis-Related Protein; Gossypium barbadense; Ribonuclease Activity; Incompatible Response
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.2A,
February
28,
2013
ABSTRACT: Early
stage expression of PR10 combined
with phytoalexins contributed to Verticillium wilt resistance in cotton. In order to analysis the activities of PR10 proteins during pathogens’
infection, we cloned a Verticillium-induced PR10 (GbPR10-1) gene from cotton
(Gossypium barbadense) and compared its expression patterns and domains with
other PR10 proteins.
Bioinformatics indicated that GbPR10-1
showed the lowest similarity with other 12 different PR10 genes in cotton (Upland and sea-island cotton). Expression profiles showed that GbPR10-1 gene instantly up-regulated
after infection by V. dahliae in the sea-island cotton plants. GbPR10-1 was also induced by
environmental stimulus including heat, submergence and salt, and ethylene but
not by ABA and salicylic acid. The GbPR10-1
protein expressed in E. coli BL21 demonstrated that it had a low
ribonuclease-like activity in vitro, and could inhibit V. dahliae hyphae growth but not its spores.
Comparison analysis of GbPR10-1 (from
resistant species) and GhPR10-1 (from
susceptible species) responding to V. dahliae infection, only GbPR10-1 gene was strongly induced in
the sea-island cotton plants (incompatible response), indicating that PR10-1 genes
was linked to resistance signal. In summary, the earlier activation of GbPR10-1 gene, as the index of
resistance response, would be aid to block the