TITLE:
Insight to the Mode of Action of Allium sativum Leaf Agglutinin (ASAL) Expressing in T3 Rice Lines on Brown Planthopper
AUTHORS:
Arpita Bala, Amit Roy, Niranjana Behura, Daniel Hess, Sampa Das
KEYWORDS:
Allium sativum leaf agglutinin (ASAL); Hemipteran Pest Management; Brown Planthopper; NADH Qui-none Oxidoreductase (NQO); Fecundity; Transgenic Rice; Brush Boarder Membrane Vesicle (BBMV) Protein
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.2A,
February
28,
2013
ABSTRACT: Brown
planthopper, the sap sucking hemipteran pest, is one of the major contributors
to the yield loss of rice through the world. To combat the situation
researchers are interested identifying genes from plant origin having potentiality
to develop hemipteran pest resistance. Interestingly, it was observed that rice
plants expressing ASAL, a monocot mannose binding lectin, showed significant
resistance to brown planthopper and green leafhopper. Additionally, antibiotic
resistant marker gene free ASAL expressing rice lines were developed to
overcome the biosafety issues. However, the basis behind the
resistance against planthoppers is still not clearly understood. Ligand blot
assay was performed with total BBMV protein from BPH and a ~56 kDa receptor
protein was detected. LC MS/MS analysis revealed that the receptor protein is
NADH quinone oxidoreductase (NQO), a key player in electron transport chain,
insect defense response and male/female gametogenesis. Presumably interaction
of ASAL with NQO may lead to toxicity and loss of fecundity among BPH feeding
on ASAL expressing transgenic rice plants. These findings provide a stable scientific
basis for considering these transgenic ASAL expressing rice plants as
significant product for combating BPH attack associated yield loss of rice.