TITLE:
Synergistic effect between cryptotanshinone and antibiotics in oral pathogenic bacteria
AUTHORS:
Jeong-Dan Cha, Mi-Ran Jeong, Kyung-Min Choi, Jeong-Hye Park, Su-Mi Cha, Kyung-Yeol Lee
KEYWORDS:
Cryptotanshinone; Antibacterial Activity; Oral Bacteria; Checker Board Method; Time-Kill Method; Synergistic Effect
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.2A,
February
28,
2013
ABSTRACT: Cryptotanshinone
(CT), a major tanshinone of medicinal plant Salvia miltiorrhiza Bunge,
demonstrated effective in vitro antibacterial activity against all oral
bacteria tested in this experiment. The antibacterial activities of CT against
oral bacteria were assessed using the checkerboard and time-kill methods
to evaluate the synergistic effects of treatment with ampicillin or
gentamicin. The CT was determined against oral pathogenic bacteria with MIC and
MBC values ranging from 0.5 to 16 and 1 to 64 μg/mL; for am- picillin from
0.0313 to 16 and 0.125 to 32 μg/mL; for gentamicin from 2 to 256 and 4 to 512
μg/mL respectively. The range of MIC50 and MIC90 were
0.0625 - 8 μg/mL and 1 - 64 μg/mL, respectively. The combination effects of
CT with antibiotics were synergistic (FIC index Streptococcus
sobrinus, S. criceti, and Actinobacillus actinomycetemcomitans (FIC index . Furthermore, a
time-kill study showed that the growth of the tested bacteria was completely attenuated after 3 - 6 h of treatment with the 1/2 MIC of CT, regardless of whether
it was administered alone or with ampicillin
or gentamicin. The results suggest that CT could be employed as a
natural antibacterial agent against cariogenic and periodontopathogenic bac- teria.