TITLE:
Development of a Performant Method for Glucocapparin Determination in Boscia senegalensis Lam Ex. Poir.: A Study of the Variability
AUTHORS:
Momar Talla Gueye, Dogo Seck, Abdoulaye Diallo, Danny Trisman, Christophe Fischer, Jean-Paul Barthelemy, Jean-Paul Wathelet, Georges Lognay
KEYWORDS:
Boscia Senegalensis; Validation Method; Glucocapparin; Monitoring; Senegal
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.4 No.2,
February
27,
2013
ABSTRACT: This study describes a glucocapparin determination
method. Based on rapeseed determination of glucosinolate (GSL), the equation of
the average straight regression line is Y = 100.42X – 0.03 (R2 =
0.9998). Enzymatic hydrolysis of glucocapparin extracted from leaves and fruits of B. senegalensis, analyzed by
SPME-GC-MS confirmed the presence of methylisothiocyanate as the main
hydrolysis glucocapparin product. Monitoring glucocapparin contents in B. senegalensis leaves and fruits collected in
4 localities in Senegal showed differences between organs according localities
and periods of harvest. Glucocapparin content was very high in dry season
particularly in January and the lowest rates were recorded during the rainy
period between August and November.