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Wilson, K. (1990) Preparation of genomic DNA from bacteria, p. 2.4.1-2.4.2. In: Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Struhl, K. (Eds.), Current Protocols in Molecular Biology, John Wiley & Sons, Inc., New York.

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• TITLE: Identification of water-borne bacterial isolates for potential remediation of organophosphate contamination

  AUTHORS: Rupa Iyer, Brian Iken

  KEYWORDS: Paraoxon Degradation; Methyl Parathion Degradation; Water Contamination; OP Pesticide

  JOURNAL NAME: Advances in Biological Chemistry, Vol.3 No.1, February 26, 2013

  ABSTRACT: Three water-borne bacterial isolates were collected from the Houston metropolitan area. Each isolate was capable of growing upon carbon limited media inoculated with the organophosphorus (OP) compound paraoxon. All isolates were able to efficiently metabolize paraoxon and, to a lesser degree, methyl parathion to p-nitrophenol. 16S rDNA genome sequencing with universal bacterial primers identified the isolates as species belonging to the genera Aeromonas, Steno- trophomonas, or Exiguobacterium. All screened isolates harbor organophosphorus degradation (opd) genes that are approximately 99% similar over approximately 660 base pairs sequenced to one first isolated from Sphingobium fuliginis ATCC 27551 (formerly Flavobacterium sp. ATCC 27551). Additionally, two isolates KKWT11, identified as a putative Senotro- phomonas maltophilia, and KKBO11, identified as a putative Exiguobacterium indicum, were found to possess genomic DNA that closely matched a metallo- beta-lactamase that has been reported to function as a methyl parathion degradation (mpd) gene suggesting that both of these strains are prime candidates for wastewater remediation of a broad range of OP compounds.