TITLE:
HFE Gene Mutation Associated with the Severity of Gestational Diabetes Mellitus in Belarusian Women
AUTHORS:
Larysa Sivitskaya, Nina Danilenko, Zoya Zabarouskaya, Oleg Davydenko
KEYWORDS:
Gestational Diabetes Mellitus; HFE Gene; H63D and C282Y Mutations; Belarusian Population
JOURNAL NAME:
Open Journal of Endocrine and Metabolic Diseases,
Vol.3 No.1,
February
25,
2013
ABSTRACT: To determine whether the H63D and C282Y mutations in HFE (hemochromatosis) gene are associated with the risk of gestational diabetes mellitus (GDM), we conducted the study of 65 incident cases. The class of gestational diabetes (A1, A2, B) in pregnant women was defined based on the results of glycemic profile and 75-g oral glucose tolerance test. Two single nucleotide polymorphisms (H63D and C282Y) in HFE gene were genotyped by PCR and RFLP (Restriction Fragment Length Polymorphism). The frequencies of mutations in patients cohort were: 0.14 for H63D and 0.02 for C282Y, which are similar to the data reported for Belarusian population (0.16 and 0.04 respectively). The detailed analysis of case subjects indicated association of H63D mutation with the severity of gestational diabetes mellitus. In the frequencies of H63D mutation and genotypes between the case subjects with A1 and B gestational diabetes were detected significant differences. Our data indicated that the presence of H63D mutation in pregnant women with GDM aggravates the disease—odds ratio 7.4 (95% CI 1.8 - 30.5). Women with gestational diabetes have severe increased risk for illness progressing to class B if they are H63D mutation carriers.