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Hayakawa, M., Otoguro, M., Takeuchi, T., Yamazaki, T. and Iimura, Y. (2000) Application of a method incorporating differential centrifugation for selective isolation of motile actinomycetes in soil and plant litter. Antonie van Leeuwenhoek, 78, 171-185. doi:10.1023/A:1026579426265

has been cited by the following article:

• TITLE: In vitro antagonistic potential of Streptomyces sp. AM-S1 against plant and human pathogens

  AUTHORS: Kandhasamy Sowndhararajan, Sun Chul Kang

  KEYWORDS: Actinomycetes; Streptomyces sp. AM-S1; Antagonistic Activity; Rhizoctonia solani

  JOURNAL NAME: Journal of Agricultural Chemistry and Environment, Vol.1 No.1, November 29, 2012

  ABSTRACT: In the present investigation, a total number of 132 different actinomycetes strains were isolated from the humus soil samples. Out of 132 isolates, 52 showed inhibitory activity against the fungal pathogen Rhizoctonia solani. Among the antagonists, the isolate designated as AM-S1 exhibited maximum inhibitory activity against the test pathogen R. solani (41 mm). Further, the light microscopic observations of the co-cultures showed severe structural alterations in the mycelia of R. solani near the zone of inhibition. The isolate AM-S1 was identified as Streptomyces sp. by morphological and 16S rDNA sequence analysis. The color of the aerial and substrate mycelia produced by the Streptomyces sp. AM-S1 varied with different media. The isolate Streptomyces sp. AM-S1 also effectively inhibited the growth of various plant and human pathogens. Further works are needed on optimization of this strain’s antagonistic activity, isolation and characterization of the antimicrobial metabolite.