TITLE:
Bovine β-casein: Detection of two single nucleotide polymorphisms by bidirectional allele specific polymerase chain reaction (BAS-PCR) and monitoring of their variation
AUTHORS:
Stefania Chessa, Omar Bulgari, Attilio Rossoni, Gabriella Ceriotti, Anna Maria Caroli
KEYWORDS:
Bovine β-Casein; Milk Protein Polymorphism
JOURNAL NAME:
Open Journal of Animal Sciences,
Vol.3 No.1,
January
29,
2013
ABSTRACT: Due
to the functional importance of bovine milk protein polymorphisms, their
correct discrimination is of great interest both from a scientific and practical
point of view. Nowadays a large number of
commercial platforms are available for semiautomated or fully automated
SNP geno-typing. However, in some cases the use of simple and rather cheap
methods is an effective tool to be implemented within one’s own laboratory for
the routine analysis of a specific SNP. The present paper describes two simple
tests based on the bidirectional allele-specific polymerase chain reaction
(BAS-PCR) developed for the identification of β-casein (CSN2) B and I genetic variants. The practical application of the two methods on
a panel of 84 Italian Brown bulls and 100 Italian Friesian cows is also discussed,
including the biological significance of the two genetic variants and the importance of taking their occurrence
into account when linkage analyses are performed on milk functional properties.
A combined system for analysing milk protein
variants by isoelectrofocusing (IEF) and the BAS-PCR assay developed
for CSN2*I is described.