TITLE:
Separation of Enantiomers of Clopidogrel on Chiral Stationary Phases by Packed Column Supercritical Fluid Chromatography
AUTHORS:
Khagga Bhavyasri, D. Rambabu, P. S. S. Prasad, V. Murali Balaram
KEYWORDS:
Chiral Stationary Phases; SFC; Enantiomer Separation; Supercritical Fluid Chromatography; Clopidogrel
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.4 No.1,
January
22,
2013
ABSTRACT: A packed column supercritical fluid chromatography (SFC) method for the
separation of clopidogrel enantiomers on a chiral stationary phase and CO2 with modifier as mobile phase has been developed at an analytical scale. Among
11 different 2 stationary phases the Chiral cel OD-H column showed by far the
best separation properties. The influence of different modifiers, injection
solvents, temperature, and pressure, and density of the fluid, respectively, on
the separation behaviour
has been studied. It was found that the separation behaviour strongly depends
on the type of modifier and the modifier content. Temperature and pressure are
of less influence.