TITLE:
Chilean crude extract of Ruta graveolens generates vasodilatation in rat aorta at subtoxic cellular concentrations
AUTHORS:
Ignacio Jofré Fernández, Patricia Navarrete Gómez, Jorge Parodi, Fernando Romero Mejía, Raúl Salvatici Salazar
KEYWORDS:
Vasodilatation; Ruta graveolens; Toxicity, Endothelium
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.1,
January
21,
2013
ABSTRACT: In Chile elevated percentage of population have cardiovascular diseases, 70% of this
populations is a hypertensive peoples. The Ruta graveolens (Ruta) is a medicinal plant used in different parts
of the world with different therapeutics properties like dermatologic as far
as anti-helmintic properties. We analyzed the vascular action measuring the
tension to identify vasodilator effect of Ruta extract in norm-tense rat’s
aorta incubated and measured in isolated organ bath, and evaluating the
toxicity effect in CRL-1730 cell line, through enzymatic assay (MTT), confocal
microscopy (propidium iodide stain) and flow cytometry (TUNEL assay), including
extracellular reactive oxygen species (ROS) production through luminescence
assay. The results show with DE50 29 ± 0.1 μg/mL evidenced
vasodilatation, partially endothelium-depend. The cytotoxicity showed with DE50 304.6 ± 2 μg/mL in enzymatic assay (MTT) while evidenced membrane
permeability in high concentrations (1500 μg/mL), DNA fragmentation in absence
of oxidative stress in only observed when high concentrations of Ruta are
used over the cell culture. The vasodilatation activity is executed in subtoxic
concentration and partially endothelium-depend without permeability effect in
the membrane and deterioration of the cells viability suggesting a complex
effect of Ruta preparation in the regulation of vascular tone.