TITLE:
Development and Validation of New RP-HPLC Method for the Estimation of Alfuzosin Hydrochloride in Bulk and Tablet Dosage Form
AUTHORS:
Vandana P. Patil, Subhash J. Devdhe, Suwarna H. Kale, Vijay J. Nagmoti, Sadanand D. Kurhade, Yuvraj R. Girbane, Mahesh T. Gaikwad
KEYWORDS:
Alfuzosin HCl; RP-HPLC; Linearity; Precision; Accuracy; Robustness; Ruggedness
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.4 No.1,
January
18,
2013
ABSTRACT: A reverse phase high
performance liquid chromatographic method (HPLC) has been developed for the
estimation of Alfuzosin hydrochloride (ALH)
in the pharmaceutical formulation using RP-C18 column. The mobile phase (Tetrahydrofuran, Acetonitrile and buffer (pH 3.50) was pumped at a flow rate of 1.5 ml/min in the ratio of 1:20:80 and
the eluents were monitored at 254.0 nm. Linearity was obtained in the
concentration range of 80 - 120 μg/mL for ALH.
The method was statistically validated and RSD was found to be less than 2%
indicating high degree of accuracy and precision of the proposed HPLC method.
Due to its simplicity, rapidness, high precision and accuracy, the proposed
HPLC method may be used for determination of Alfuzosin hydrochloride in bulk
drugs and in pharmaceutical dosage form.