TITLE:
In Vitro Study of Carbamate-Linked Cationic Lipid for Gene Delivery Against Cervical Cancer Cells
AUTHORS:
Defu Zhi, Shuibao Zhang, Yinan Zhao, Shaohui Cui, Bing Wang, Huiying Chen, Defu Zhi, Defeng Zhao
KEYWORDS:
Cationic Lipids; DNA Condensation; Gene Delivery; Transfection Efficiency
JOURNAL NAME:
Advances in Materials Physics and Chemistry,
Vol.2 No.4B,
January
17,
2013
ABSTRACT: Design and synthesis of a carbamate-linked cationic lipid DDCTMA (N-[1-(2,3-didodecylcarbamoyloxy)propyl]-N,N,N-trimethylammonium iodide) as gene delivery carriers was described in this work. The transfection efficiency of cationic liposome increased dramatically with the increase in the content of DOPE. In addition, the transfection efficiency of some of cationic lipoplexes was superior or parallel to that of two commercial transfection agents, Lipofectamine2000 and DOTAP. The carbamate-linked cationic lipid DDCTMA/DOPE may be a promising gene carrier that has high transfection efficiency as well as low cytotoxicity.