TITLE:
Redox Factor-1 Mediates Inflammatory Response during Tumor Promotion in Skin Epidermal JB6 Cells
AUTHORS:
Delira Robbins, Wenjuan Li, Katie Humphrey, Yunfeng Zhao
KEYWORDS:
Ref-1; Tumorigenesis; JB6 Cells; Tumor Promotion; Chemoprevention
JOURNAL NAME:
Open Journal of Apoptosis,
Vol.1 No.3,
October
26,
2012
ABSTRACT: Recently, redox factor-1 (Ref-1) has received considerable attention as an enzyme for stimulating tumor cell growth. We hypothesized that Ref-1 is upregulated during the early stage of tumorigenesis. Utilizing both tumor promotion sensitive P+ and promotion resistant P? mouse skin epidermal JB6 cells, we found that Ref-1 expression was only induced in tumor promotion sensitive P+ cells following TPA treatment. Consistent with that, Ref-1 knockdown suppressed skin cell transformation. Interestingly, Ref-1 knockdown suppressed nuclear translocation of NF-kappaB subunit p65, and inhibited production of proinflammatory cytokines. These results suggest Ref-1 may promote early tumorigenesis and serve as a target for chemoprevention.