TITLE:
Effect of Hot Water Extract from Agaricus Blazei Murill on Chemotaxis of Neutrophils
AUTHORS:
Mayuko Miyagawa, Yuriko Hirono, Ayaka Kawazoe, Eri Shigeyoshi, Masahito Nose, Masaaki Sakura, K. E. Pinkerton, Minoru Takeuchi
KEYWORDS:
Neutrophil; Chemotactic Activity; Phagocytic Activity; ROS Production; Agaricus Blazei Murill
JOURNAL NAME:
Journal of Cosmetics, Dermatological Sciences and Applications,
Vol.3 No.1A,
January
10,
2013
ABSTRACT: Hot water extract from the edible
Brazilian mushroom, Agaricus Blazei Murill (ABM), is used for both traditional
and alternative medicine. ABM is
reported to stimulate anti-tumor, anti-infection, and immune activity. However,
there are few reports of how ABM affects neutrophils. Therefore, in this study,
we examined the effect of hot water ABM extract on neutrophil migration,
phagocytosis, and reactive oxygen species production using neutrophils from
guinea pig. Migratory direction and velocity as indicators of chemotactic
activity of neutrophils were significantly (p 0.001) increased at concentration of
50 and 100 mg/ml in ABM extract compared with control. Phagocytic activity of
neutrophil was significantly (p 0.01) increased at
concentration of 5 mg/ml in ABM extract compared with control. Production of reactive
oxygen species (ROS: H2O2 or ) by neutrophils was
significantly (p 0.01) increased at
concentration of 5 mg/ml in ABM extract compared with control. These results
suggest that enhancement in neutrophil chemotactic activity, phagocytic
activity and ROS production are mechanisms by which ABM extract inhibits
bacterial infection in the skin and dermatitis.